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小鼠 anti-人 IgE Antibody

ELISA, FACS Monoclonal 4G7-325 IgG unconjugated
产品编号 ABIN94398
发货至: 中国
  • 抗原 See all IgE products
    IgE
    适用
    • 48
    • 30
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    宿主
    • 58
    • 18
    • 10
    • 10
    • 4
    • 1
    小鼠
    克隆类型
    • 66
    • 28
    单克隆
    应用范围
    • 55
    • 39
    • 30
    • 20
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS)
    特异性
    The mouse monoclonal antibody 4G7.325 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).
    交叉反应 (详细)
    Human
    纯化方法
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    Purified human IgE.
    克隆位点
    4G7-325
    亚型
    IgG
  • 应用备注
    Flow cytrometry: Recommended dilution: 1-4 μg/mL.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Smith, Benjamin, Hozic, Derewenda, Smith, Thomas, Gafvelin, van Hage-Hamsten, Chapman: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." in: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).

    Mueller, Smith, Chapman, Rule, Benjamin: "Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9359-65, (2001) (PubMed).

  • 抗原
    IgE
    Abstract
    IgE 产品
    背景
    Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
    分子量
    180 kDa
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