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Cytokeratin 19 抗体

Name: Cytokeratin 19 抗体
Brand: antibodies-online
SKU: ABIN94288
Availability: OutOfStock

KRT19 适用: 人 WB, FACS, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal BA-17 unconjugated

Detection of cytokeratin 19 in MCF-7 cell lysate by mouse monoclonal antibody BA-17 .

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody BA-17 ((ABIN94288), diluted 1:100), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

IHC (p)

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (BA-17) purified antibody (concentration in sample 3 μg/mL, GAM APC).

FACS

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 19 (BA-17).

IHC (p)

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody BA-17 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

5 images

(4 references)

产品编号 ABIN94288

发货至: 中国

Datasheet as PDF

抗原 See all Cytokeratin 19 (KRT19) 抗体

Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
适用
260

90

72

8

7

7

5

5

4

2

2

2

2

2

2

1

1

1

1

1
人
宿主
138

131

3

2

1
小鼠
Compatible Secondaries
克隆类型
143

132
单克隆
标记
123

33

20

13

7

5

5

5

5

5

5

5

4

4

4

4

4

4

3

3

3

3

3

2

2

1

1
This Cytokeratin 19 antibody is un-conjugated
应用范围
191

94

87

81

69

47

40

39

33

30

23

23

7

6

6

5

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

特异性

The antibody BA-17 reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Mammary organoids.

克隆位点

BA-17

亚型

IgG1 kappa

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KRT19 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal KRT19-799 unconjugated

anti-Keratin 19 (KRT19) (AA 241-400) antibody
KRT19 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Keratin 19 (KRT19) (AA 274-400) antibody
KRT19 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8H1A8 unconjugated

anti-Keratin 19 (KRT19) (AA 80-400) antibody
KRT19 适用: 人, 大鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2A1G9 unconjugated

应用备注

Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Samluk, Zakrzewska, Pluta: "Generation of Fluorescently Labeled Cell Lines, C3A Hepatoma Cells, and Human Adult Skin Fibroblasts to Study Coculture Models." in: Artificial organs, (2013) (PubMed).

Lewis, Klimstra, Varmus: "The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer." in: Genes & development, Vol. 17, Issue 24, pp. 3127-38, (2003) (PubMed).

Bartek, Taylor-Papadimitriou, Miller, Millis: "Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 299-306, (1985) (PubMed).

Bartek, Durban, Hallowes, Taylor-Papadimitriou: "A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins." in: Journal of cell science, Vol. 75, pp. 17-33, (1985) (PubMed).
抗原

Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

别名

Cytokeratin 19 (KRT19 产品)

别名

CK19 antibody, K19 antibody, K1CS antibody, AI663979 antibody, EndoC antibody, Krt-1.19 antibody, Krt1-19 antibody, Ka19 antibody, k19 antibody, ck19 antibody, k1cs antibody, krt9 antibody, krt15 antibody, MGC76282 antibody, GK-19 antibody, MGC83069 antibody, KRT19 antibody, keratin 19 antibody, keratin 19 L homeolog antibody, keratin, type I cytoskeletal 19 antibody, KRT19 antibody, Krt19 antibody, krt19 antibody, krt19.L antibody, LOC100344434 antibody, LOC101117946 antibody

背景

Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

基因ID

3880

UniProt

P08727