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TUBB3 抗体 (N-Term)

TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal TU-20 unconjugated
产品编号 ABIN93911
发货至: 中国
  • 抗原 See all TUBB3 抗体
    TUBB3 (Tubulin, beta 3 (TUBB3))
    抗原表位
    • 21
    • 16
    • 15
    • 12
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    适用
    • 126
    • 69
    • 62
    • 20
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, 犬, Pig
    宿主
    • 81
    • 49
    • 4
    • 1
    • 1
    小鼠
    克隆类型
    • 71
    • 65
    单克隆
    标记
    • 81
    • 14
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TUBB3 antibody is un-conjugated
    应用范围
    • 110
    • 41
    • 40
    • 36
    • 29
    • 27
    • 25
    • 16
    • 15
    • 14
    • 13
    • 6
    • 5
    • 4
    • 4
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    特异性
    The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
    交叉反应 (详细)
    Broad species reactivity
    纯化方法
    Purified by protein-A affinity chromatography.
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
    克隆位点
    TU-20
    亚型
    IgG1
  • 应用备注
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: porcine brain lysate, negative control: HPB-ALL human peripheral blood leukemia cell line, reducing conditions.
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, standard ABC technique (DAB+), pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or high temperature citrate buffer antigen retrieval, positive tissue: neuronal tissue.
    Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line.
    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Letellier, Szíber, Chamma, Saphy, Papasideri, Tessier, Sainlos, Czöndör, Thoumine: "A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation." in: Nature communications, Vol. 9, Issue 1, pp. 3979, (2019) (PubMed).

    Gudernova, Balek, Varecha, Kucerova, Kunova Bosakova, Fafilek, Palusova, Uldrijan, Trantirek, Krejci: "Inhibitor repurposing reveals ALK, LTK, FGFR, RET and TRK kinases as the targets of AZD1480." in: Oncotarget, Vol. 8, Issue 65, pp. 109319-109331, (2017) (PubMed).

    Szíber, Liliom, Morales, Ignácz, Rátkai, Ellwanger, Link, Szűcs, Hausser, Schlett: "Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover." in: Molecular biology of the cell, Vol. 28, Issue 2, pp. 285-295, (2017) (PubMed).

    Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

    Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, (2011) (PubMed).

    Kunova, Matulka, Eiselleova, Trckova, Hampl, Dvorak: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, (2010) (PubMed).

    Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

    Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, (2009) (PubMed).

    Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

    Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

    Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, (2007) (PubMed).

    Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).

    P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, (2001) (PubMed).

    Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).

    Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).

  • 抗原
    TUBB3 (Tubulin, beta 3 (TUBB3))
    别名
    betaIII-tubulin (TUBB3 产品)
    别名
    CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, TUBB3 antibody, 3200002H15Rik antibody, M(beta)3 antibody, M(beta)6 antibody, tubb3 antibody, tubulin beta 3 class III antibody, tubulin, beta 3 class III antibody, tubulin beta 3 class III L homeolog antibody, beta-tubulin 3 antibody, tubulin beta-3 chain antibody, beta tubulin 3 antibody, TUBB3 antibody, Tubb3 antibody, tubb3 antibody, tubb3.L antibody, LOC100581245 antibody, tub3 antibody
    背景
    Tubulin beta 3,The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.,TUBB3
    基因ID
    10381
    UniProt
    Q13509
    途径
    Microtubule Dynamics, M Phase
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