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RBCL (Isoform 1) 抗体

适用: Arabidopsis thaliana, Lobaria pulmonaria, Pisum sativum, Potato, Spinach WB, IL 宿主: 小鸡 Polyclonal unconjugated
产品编号 ABIN93524
发货至: 中国
  • 抗原
    RBCL
    抗原表位
    • 8
    • 4
    • 1
    • 1
    • 1
    Isoform 1
    适用
    Arabidopsis thaliana, Lobaria pulmonaria, Pisum sativum, Potato, Spinach
    宿主
    • 14
    小鸡
    克隆类型
    • 14
    多克隆
    标记
    • 5
    • 3
    • 3
    • 3
    非结合性
    应用范围
    • 10
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunolocalization (IL)
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
    纯化方法
    affinity purified
    免疫原
    KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
  • 应用备注
    Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 2 µg of total cellular protein, detected with standard ECL(WB), (IL) tested on a grass species, formaldehyde-fixed and paraffin-embeddedtissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116.
    说明

    This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

    限制
    仅限研究用
  • 状态
    Liquid
    溶解方式
    For reconstitution add 225 µL of milliQ water per each tube
    浓度
    16 µg/µL
    缓冲液
    PBS pH 8.0+ 0.02 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Make aliquots to avoid working with a stock.
    Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.
    储存条件
    4 °C
  • Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

    Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

    Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

  • 抗原
    RBCL
    背景
    AGI Code: ATCG00490
    Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
    分子量
    52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
    UniProt
    Q3ALL1, O03042
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