Myc 标记 抗体

ABIN933570 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Dot Blot (DB), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Myc Tag antibody was raised in mouse using EQKLISEEDL (Myc) synthetic peptide conjugated to KLH as the immunogen.
序列 EQKLISEEDL
克隆位点 Myc-A7
亚型 IgG1
纯化方法 Protein A affinity chromatography
物质类 Tag
背景 A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis. Synonyms: Monoclonal Myc Tag antibody, anti-Myc Tag antibody, Myc antibody, C-myc antibody, Cmyc antibody.
研究领域 Tags/Labels
应用备注 WB: 1:1000-3000
Optimal conditions should be determined by the investigator.
限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
储存液 Sodium azide
注意事项 This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
储存条件 RT/-20 °C
储存方法 Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
厂商提供的图像
Western Blotting (WB) image for anti-Myc Tag antibody (ABIN933570) 1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with Myc-...
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