(1 reference)-
- 抗原 See all CDC25B 抗体
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
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抗原表位
- pSer149
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适用
- 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDC25B antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
- 纯化方法
- Affinity purified
- 免疫原
- Peptide sequence around phosphorylation site of pSer149 (F-R-S (p) -L-P) derived from Mouse Cdc25B. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
- Top Product
- Discover our top product CDC25B Primary Antibody
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anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer186) antibody
CDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-195) antibodyCDC25B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 331-360) antibodyCDC25B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07936 unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) antibodyCDC25B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Western blotting: 1:500-1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs." in: The Journal of biological chemistry, Vol. 286, Issue 12, pp. 10356-66, (2011) (PubMed).
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: "Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs." in: The Journal of biological chemistry, Vol. 286, Issue 12, pp. 10356-66, (2011) (PubMed).
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- 抗原
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
- 别名
- Cdc25B (CDC25B 产品)
- 别名
- CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody
- 背景
- Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
- 分子量
- 62 kDa
- 基因ID
- 12531
- NCBI登录号
- NP_001104
- UniProt
- Q9DBN8
- 途径
- Cell Division Cycle, M Phase, Autophagy
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