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SCNN1A 抗体 (AA 617-638)

Name: SCNN1A 抗体 (AA 617-638)
Brand: antibodies-online
SKU: ABIN863203
Availability: OutOfStock

SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (ABIN863203).

The expression of ENaC subunits after preservation in UW solution.

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody (ABIN863203).

IHC

Western blot analysis of Mouse mpkCCD cell lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (ABIN863203).

5 images

(19 references)

产品编号 ABIN863203

发货至: 中国

Datasheet as PDF

抗原 See all SCNN1A 抗体

SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))
抗原表位
63

22

20

11

10

7

2

1

1

1

1

1

1

1

1

1

1
AA 617-638
适用
99

45

39

20

10
大鼠
宿主
82

43
兔
Compatible Secondaries
克隆类型
82

43
多克隆
标记
27

11

10

10

10

10

9

9

9

9

5

1

1

1

1

1

1
This SCNN1A antibody is un-conjugated
应用范围
115

103

36

35

33

21

10

4

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

Detects ~87 kDa.

交叉反应

Hamster, 人, 小鼠, 大鼠, 非洲爪蟾

纯化方法

Protein A Purified

免疫原

Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 629-650) antibody
SCNN1A 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 46-68) antibody
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) antibody
SCNN1A 适用: 大鼠, 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 201-300) antibody
SCNN1A 适用: 大鼠, 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 617-638) antibody (HRP)
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal HRP

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 617-638) antibody (FITC)
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal FITC

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 617-638) antibody (Alkaline Phosphatase (AP))
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 617-638) antibody (Biotin)
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal Biotin

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 617-638) antibody (PE)
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal PE

anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 617-638) antibody (APC)
SCNN1A 适用: 大鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal APC

应用备注
- WB (1:1000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN863203 was sufficient for detection of beta-ENaC in 20 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Khedr, Palygin, Pavlov, Blass, Levchenko, Alsheikh, Brands, El-Meanawy, Staruschenko: "Increased ENaC activity during kidney preservation in Wisconsin solution." in: BMC nephrology, Vol. 20, Issue 1, pp. 145, (2020) (PubMed).

Blass, Klemens, Brands, Palygin, Staruschenko: "Postprandial Effects on ENaC-Mediated Sodium Absorption." in: Scientific reports, Vol. 9, Issue 1, pp. 4296, (2019) (PubMed).

Pavlov, Levchenko, Ilatovskaya, Moreno, Staruschenko: "Renal sodium transport in renin-deficient Dahl salt-sensitive rats." in: Journal of the renin-angiotensin-aldosterone system : JRAAS, Vol. 17, Issue 3, (2017) (PubMed).

Zhang, Sun, Ding, Huang, Zhang, Jia: "Inhibition of Mitochondrial Complex-1 Prevents the Downregulation of NKCC2 and ENaCα in Obstructive Kidney Disease." in: Scientific reports, Vol. 5, pp. 12480, (2015) (PubMed).

Vitzthum, Seniuk, Schulte, Müller, Hetz, Ehmke: "Functional coupling of renal K+ and Na+ handling causes high blood pressure in Na+ replete mice." in: The Journal of physiology, Vol. 592, Issue Pt 5, pp. 1139-57, (2014) (PubMed).

Collier, Tomkovicz, Peterson, Benson, Snyder: "Intersubunit conformational changes mediate epithelial sodium channel gating." in: The Journal of general physiology, Vol. 144, Issue 4, pp. 337-48, (2014) (PubMed).

Xu, Barone, Brooks, Soleimani: "Double knockout of carbonic anhydrase II (CAII) and Na(+)-Cl(-) cotransporter (NCC) causes salt wasting and volume depletion." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 7, pp. 173-83, (2014) (PubMed).

Hye Khan, Pavlov, Christain, Neckář, Staruschenko, Gauthier, Capdevila, Falck, Campbell, Imig: "Epoxyeicosatrienoic acid analogue lowers blood pressure through vasodilation and sodium channel inhibition." in: Clinical science (London, England : 1979), Vol. 127, Issue 7, pp. 463-74, (2014) (PubMed).

Davies, Fraser, Galic, Choy, Katerelos, Gleich, Kemp, Mount, Power: "Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK." in: American journal of physiology. Renal physiology, Vol. 307, Issue 1, pp. F96-F106, (2014) (PubMed).

Ramkumar, Stuart, Rees, Hoek, Sigmund, Kohan: "Collecting duct-specific knockout of renin attenuates angiotensin II-induced hypertension." in: American journal of physiology. Renal physiology, Vol. 307, Issue 8, pp. F931-8, (2014) (PubMed).

Pavlov, Levchenko, Staruschenko: "Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption." in: The Journal of biological chemistry, Vol. 289, Issue 41, pp. 28651-9, (2014) (PubMed).

Roos, Bugaj, Mironova, Stockand, Ramkumar, Rees, Kohan: "Adenylyl cyclase VI mediates vasopressin-stimulated ENaC activity." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 2, pp. 218-27, (2013) (PubMed).

Pavlov, Ilatovskaya, Levchenko, Li, Ecelbarger, Staruschenko: "Regulation of ENaC in mice lacking renal insulin receptors in the collecting duct." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 7, pp. 2723-32, (2013) (PubMed).

Miller, Loewy: "ENaC γ-expressing astrocytes in the circumventricular organs, white matter, and ventral medullary surface: sites for Na+ regulation by glial cells." in: Journal of chemical neuroanatomy, Vol. 53, pp. 72-80, (2013) (PubMed).

Soleimani, Barone, Xu, Shull, Siddiqui, Zahedi, Amlal: "Double knockout of pendrin and Na-Cl cotransporter (NCC) causes severe salt wasting, volume depletion, and renal failure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 33, pp. 13368-73, (2012) (PubMed).

van der Lubbe, Lim, Meima, van Veghel, Rosenbaek, Mutig, Danser, Fenton, Zietse, Hoorn: "Aldosterone does not require angiotensin II to activate NCC through a WNK4-SPAK-dependent pathway." in: Pflügers Archiv : European journal of physiology, Vol. 463, Issue 6, pp. 853-63, (2012) (PubMed).

Yu, Thelin, Rogers, Stutts, Randell, Grubb, Boucher: "Regional differences in rat conjunctival ion transport activities." in: American journal of physiology. Cell physiology, Vol. 303, Issue 7, pp. C767-80, (2012) (PubMed).

Edinger, Bertrand, Rondandino, Apodaca, Johnson, Butterworth: "The epithelial sodium channel (ENaC) establishes a trafficking vesicle pool responsible for its regulation." in: PLoS ONE, Vol. 7, Issue 9, pp. e46593, (2012) (PubMed).

Ilatovskaya, Pavlov, Levchenko, Negulyaev, Staruschenko: "Cortical actin binding protein cortactin mediates ENaC activity via Arp2/3 complex." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 8, pp. 2688-99, (2011) (PubMed).
抗原

SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

别名

ENaC (SCNN1A 产品)

别名

BESC2 antibody, ENaCa antibody, ENaCalpha antibody, SCNEA antibody, SCNN1 antibody, ENaC antibody, Scnn1 antibody, mENaC antibody, SCNN1A antibody, alphaxENaC antibody, besc2 antibody, enaca antibody, scnn1 antibody, ENAC antibody, sodium channel epithelial 1 alpha subunit antibody, sodium channel, nonvoltage-gated 1 alpha antibody, sodium channel, non voltage gated 1 alpha subunit L homeolog antibody, Scnn1a antibody, SCNN1A antibody, scnn1a.L antibody

背景

The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

基因ID

24767

NCBI登录号

NP_036780

UniProt

P37090