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SLC12A3 抗体 (AA 74-95)

SLC12A3 适用: 大鼠 WB, IHC, ICC, IF, IEM 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN863201
发货至: 中国
  • 抗原 See all SLC12A3 抗体
    SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
    抗原表位
    • 16
    • 10
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 74-95
    适用
    • 34
    • 13
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    大鼠
    宿主
    • 42
    • 4
    克隆类型
    • 42
    • 4
    多克隆
    标记
    • 11
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SLC12A3 antibody is un-conjugated
    应用范围
    • 25
    • 16
    • 14
    • 14
    • 13
    • 13
    • 12
    • 10
    • 9
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)
    特异性
    Detects ~160 kDa.
    交叉反应
    犬, 人, 小鼠, 大鼠
    纯化方法
    Affinity Purified
    免疫原
    Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
  • 应用备注
    • WB (1:1000)
    • IHC (1:200)
    • optimal dilutions for assays should be determined by the user.
    说明

    1 μg/ml of ABIN863201 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Fenton, Poulsen, de la Mora Chavez, Soleimani, Dominguez Rieg, Rieg: "Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis." in: Kidney international, Vol. 92, Issue 2, pp. 397-414, (2018) (PubMed).

    Procino, Carmosino, Milano, Dal Monte, Schena, Mastrodonato, Gerbino, Bagnoli, Svelto: "β3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function." in: Kidney international, Vol. 90, Issue 3, pp. 555-67, (2017) (PubMed).

    Patel-Chamberlin, Varasteh Kia, Xu, Barone, Zahedi, Soleimani: "The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150918, (2016) (PubMed).

    Gois, Canale, Luchi, Volpini, Veras, Costa, Shimizu, Seguro: "Tenofovir during pregnancy in rats: a novel pathway for programmed hypertension in the offspring." in: The Journal of antimicrobial chemotherapy, Vol. 70, Issue 4, pp. 1094-105, (2015) (PubMed).

    Wen, Yuan, Warner, Wang, Cornelius, Wang-France, Li, Boettger, Sansom: "Increased Epithelial Sodium Channel Activity Contributes to Hypertension Caused by Na+-HCO3- Cotransporter Electrogenic 2 Deficiency." in: Hypertension, Vol. 66, Issue 1, pp. 68-74, (2015) (PubMed).

    Cheng, Wu, Kortenoeven, Pisitkun, Fenton: "A Systems Level Analysis of Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells." in: Scientific reports, Vol. 5, pp. 12829, (2015) (PubMed).

    Rosenbaek, Kortenoeven, Aroankins, Fenton: "Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13347-61, (2014) (PubMed).

    Esteva-Font, Guillén-Gómez, Diaz, Guirado, Facundo, Ars, Ballarin, Fernández-Llama: "Renal sodium transporters are increased in urinary exosomes of cyclosporine-treated kidney transplant patients." in: American journal of nephrology, Vol. 39, Issue 6, pp. 528-35, (2014) (PubMed).

    Donnelly, Needham, Snyder, Roy, Khadem, Brodsky, Subramanya: "Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis." in: The Journal of biological chemistry, Vol. 288, Issue 18, pp. 13124-35, (2013) (PubMed).

    Rosenbaek, Assentoft, Pedersen, MacAulay, Fenton: "Characterization of a novel phosphorylation site in the sodium-chloride cotransporter, NCC." in: The Journal of physiology, Vol. 590, Issue Pt 23, pp. 6121-39, (2012) (PubMed).

  • 抗原
    SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
    别名
    NCC (SLC12A3 产品)
    别名
    SLC12A3 antibody, slc12a3 antibody, DKFZp469N2315 antibody, NCC antibody, NCCT antibody, TSC antibody, AI035291 antibody, solute carrier family 12 member 3 antibody, solute carrier family 12 (sodium/chloride transporter), member 3 antibody, solute carrier family 12, member 3 antibody, SLC12A3 antibody, slc12a3.2 antibody, Slc12a3 antibody
    背景
    NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
    基因ID
    54300, 6559
    NCBI登录号
    NP_062218, NP_000330
    UniProt
    P55018, P55017
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