HSPB8 抗体
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- 抗原 See all HSPB8 抗体
- HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSPB8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~22 kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- His-tagged human recombinant HSP22
- 克隆位点
- 3C12-H11
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product HSPB8 Primary Antibody
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anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody
HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 22kDa Protein 8 (HSPB8) (AA 1-196) antibodyHSPB8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 22kDa Protein 8 (HSPB8) (AA 97-196) antibodyHSPB8 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (HRP)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (FITC)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (Alkaline Phosphatase (AP))HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (Biotin)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (PE)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (PerCP)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PerCP
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (APC)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal APC
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- 应用备注
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- WB (1:2000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN863102 was sufficient for detection of HSP22 in 20 μg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))
- 别名
- HSP22 (HSPB8 产品)
- 别名
- CMT2L antibody, DHMN2 antibody, E2IG1 antibody, H11 antibody, HMN2 antibody, HMN2A antibody, HSP22 antibody, MGC64408 antibody, hsc70 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, fc09c11 antibody, wu:fc04b04 antibody, wu:fc09c11 antibody, zgc:64202 antibody, AU018630 antibody, AW413033 antibody, Cryac antibody, D5Ucla4 antibody, H11K antibody, HSP20-like antibody, Hsp22 antibody, heat shock protein family B (small) member 8 antibody, heat shock protein family B (small) member 8 L homeolog antibody, heat shock protein 8 antibody, heat shock protein b8 antibody, HSPB8 antibody, hspb8.L antibody, hspa8 antibody, hspb8 antibody, Hspb8 antibody
- 背景
- HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6).
- 基因ID
- 26353
- NCBI登录号
- NP_055180
- UniProt
- Q9UJY1
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