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WNT2 抗体 (AA 221-320)

WNT2 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN762896
发货至: 中国
  • 抗原 See all WNT2 抗体
    WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
    抗原表位
    • 14
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 221-320
    适用
    • 31
    • 21
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 30
    • 1
    克隆类型
    • 30
    • 1
    多克隆
    标记
    • 17
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    This WNT2 antibody is un-conjugated
    应用范围
    • 29
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    交叉反应
    人, 小鼠
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human WNT2
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #029629 (Immunohistochemistry)
    '独立验证'标志
    by
    Histopathology and Tissue Shared Resource, Georgetown Lombardi Comprehensive Cancer Center
    No.
    #029629
    日期
    2014.03.16
    抗原
    Lot Number
    131105
    Method validated
    Immunohistochemistry
    Positive Control
    Human glioma tissue
    Negative Control
    Human brain
    Notes
    Signal was detected in positive control tissue and not in negative control tissue.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
    • Catalog number: ABIN762896
    • Lot number: 131105
    Secondary Antibody
    • Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
    • Batch number: 10082183
    Full Protocol
    • Immunohistochemistry was performed by hand.
    • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
    • Sections were rinsed in de-ionized water for 5 min at RT.
    • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
    • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
    • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
    • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
    • Sections were rinsed in TBST for 5 min 2X at RT.
    • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
    • Sections were rinsed in TBST for 5 min 2X at RT.
    • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
    • Sections were washed x 1 in Distilled Water.
    • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were blued in Ammonium Hydroxide for 1 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
    Experimental Notes
    • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
    • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

  • 抗原
    WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
    别名
    WNT2 (WNT2 产品)
    别名
    CG1916 antibody, D-wnt-2 antibody, DWnt-2 antibody, DWnt2 antibody, Dm DWnt2 antibody, Dm-2 antibody, Dmel\\CG1916 antibody, Dwnt-2 antibody, Wnt antibody, Wnt-2 antibody, dWnt2 antibody, wnt2 antibody, WNT2 antibody, irp antibody, Xwnt2 antibody, wnt-2 antibody, Xwnt-2 antibody, int1l1 antibody, 2610510E18Rik antibody, Int1l1 antibody, Irp antibody, Mirp antibody, Wnt2a antibody, INT1L1 antibody, IRP antibody, ZfWnt2 antibody, Wnt oncogene analog 2 antibody, Wnt family member 2 antibody, wingless-type MMTV integration site family member 2 antibody, wingless-type MMTV integration site family, member 2 antibody, Wnt2 antibody, wnt2 antibody, WNT2 antibody
    背景

    Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2

    Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

    基因ID
    7472
    UniProt
    P09544
    途径
    WNT signaling
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