WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290)

Details for Product anti-WNT2 Antibody No. ABIN762896, 供应商: Log in to see
抗原
  • CG1916
  • D-wnt-2
  • DWnt-2
  • DWnt2
  • Dm DWnt2
  • Dm-2
  • Dmel\\CG1916
  • Dwnt-2
  • Wnt
  • Wnt-2
  • dWnt2
  • wnt2
  • WNT2
  • irp
  • Xwnt2
  • wnt-2
  • Xwnt-2
  • int1l1
  • 2610510E18Rik
  • Int1l1
  • Irp
  • Mirp
  • Wnt2a
  • INT1L1
  • IRP
  • ZfWnt2
  • Wnt oncogene analog 2
  • wingless-type MMTV integration site family member 2
  • wingless-related MMTV integration site 2
  • Wnt2
  • wnt2
  • LOC100221216
  • WNT2
Alternatives
anti-人 WNT2 抗体 for Immunofluorescence (fixed cells)
抗原表位
AA 240-290
14
6
3
3
2
2
2
1
1
1
1
适用
犬, 人, 小鼠, 大鼠
64
37
30
18
4
4
4
4
4
4
3
2
2
宿主

66
克隆类型
多克隆
标记
This WNT2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
40
13
12
10
5
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

'独立验证'标志
Lot Number 131105
Method validated Immunohistochemistry
Positive Control Human glioma tissue
Negative Control Human brain
Notes Signal was detected in positive control tissue and not in negative control tissue.
Primary Antibody
  • Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
  • Catalog number: ABIN762896
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131105
Isotype Control Antibody
Secondary Antibody
  • Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Batch number: 10082183
Controls
  • Positive control: Human Glioma (specimen known to contain the target protein) from HTSR’s Human Tissue Bank.
  • Negative Control: Human Normal Brain (specimen known to not contain the target protein) from HTSR’s Human Tissue Bank.
  • Primary antibody isotype control: Human Glioma treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Human Glioma treated with secondary antibody only (no primary antibody).
实验流程
  • Immunohistochemistry was performed by hand.
  • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
  • Sections were rinsed in de-ionized water for 5 min at RT.
  • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
  • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
  • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
  • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
  • Sections were washed x 1 in Distilled Water.
  • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were blued in Ammonium Hydroxide for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
Experimental Notes
  • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
  • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
Validation Images
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 1: Human brain glioma tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 2: Human normal brain tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 3: Human brain glioma tissue stained with isotype control antibody (brown) and...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 4: Human brain glioma tissue stained with secondary only (brown) and counterst...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 5: Human brain tissue with Parkinson's morphology stained with anti-Wnt2 (brow...
免疫原 KLH conjugated synthetic peptide derived from human WNT2
亚型 IgG
纯化方法 Purified by Protein A.
别名 WNT2 (WNT2 Antibody 摘要)
背景

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Subcellular location: Secreted

Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2

基因ID 7472
UniProt P09544
研究领域 Cancer, Wnt
途径 WNT signaling
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months
厂商提供的图像
Immunohistochemistry (IHC) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Formalin-fixed and paraffin embedded human placenta labeled with Anti-WNT2 Polyclonal...
Immunohistochemistry (IHC) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Immunohistochemistry (IHC) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Western Blotting (WB) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown i...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded ca...
Western Blotting (WB) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Mouse brain lysates probed with Anti-WNT2 Polyclonal Antibody, Unconjugated at 1:500...
Western Blotting (WB) image for anti-WNT2 抗体 (Wingless-Type MMTV Integration Site Family Member 2) (AA 240-290) (ABIN762896) Image provided by the Independent Validation Program (badge number 29808). Lane 1: A5...
有引用在: Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, 2014 (PubMed).