WNT2 抗体 (AA 221-320)
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- 抗原 See all WNT2 抗体
- WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
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抗原表位
- AA 221-320
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This WNT2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应
- 人, 小鼠
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human WNT2
- 亚型
- IgG
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- by
- Histopathology and Tissue Shared Resource, Georgetown Lombardi Comprehensive Cancer Center
- No.
- #029629
- 日期
- 2014.03.16
- 抗原
- Lot Number
- 131105
- Method validated
- Immunohistochemistry
- Positive Control
- Human glioma tissue
- Negative Control
- Human brain
- Notes
- Signal was detected in positive control tissue and not in negative control tissue.
- Primary Antibody
- Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
- Catalog number: ABIN762896
- Lot number: 131105
- Secondary Antibody
- Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
- Batch number: 10082183
- Full Protocol
- Immunohistochemistry was performed by hand.
- Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
- Sections were rinsed in de-ionized water for 5 min at RT.
- Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
- Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
- Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
- Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
- Sections were rinsed in TBST for 5 min 2X at RT.
- Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
- Sections were rinsed in TBST for 5 min 2X at RT.
- Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
- Sections were washed x 1 in Distilled Water.
- Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
- Sections were washed x 1 in Distilled Water.
- Sections were blued in Ammonium Hydroxide for 1 min.
- Sections were washed x 1 in Distilled Water.
- Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
- Experimental Notes
- The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
- The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
生效 #029629 (Immunohistochemistry)Validation ImagesFull Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).
: "
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Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).
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- 抗原
- WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
- 别名
- WNT2 (WNT2 产品)
- 背景
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Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2
Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
- 基因ID
- 7472
- UniProt
- P09544
- 途径
- WNT signaling
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