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LRP2 抗体 (AA 3401-3500) (Cy3)

LRP2 适用: 人, 小鼠 FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal Cy3
产品编号 ABIN750991
发货至: 中国
  • 抗原 See all LRP2 抗体
    LRP2 (Low Density Lipoprotein Receptor-Related Protein 2 (LRP2))
    抗原表位
    • 15
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 3401-3500
    适用
    • 55
    • 31
    • 16
    • 4
    • 4
    人, 小鼠
    宿主
    • 53
    • 2
    克隆类型
    • 53
    • 3
    多克隆
    标记
    • 19
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LRP2 antibody is conjugated to Cy3
    应用范围
    • 22
    • 17
    • 16
    • 13
    • 12
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    交叉反应
    人, 小鼠
    预测反应
    Rat,Dog,Horse,Chicken,Rabbit
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human Megalin
    亚型
    IgG
  • 应用备注
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #029611 (Immunofluorescence)
    '独立验证'标志
    by
    CaresBio Laboratory
    No.
    #029611
    日期
    2014.02.11
    抗原
    Lot Number
    YEYY9
    Method validated
    Immunofluorescence
    Positive Control
    MCF7 cells
    Negative Control
    HeLa cells
    Notes
    Strong signal was detected in positive control tissues and not in negative control tissues. Note that there was a small amount of signal generated in the negative control sample.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) antibody (Cy3)
    • Catalog number: ABIN750991
    • Lot number: YEYY9
    Secondary Antibody
    • Used only for the secondary only control, as primary antibody was directly conjugated to Cy3
    • Antibody: Cy3 Goat Anti-Rabbit IgG (H+L)
    Full Protocol
    • MCF7 and HeLa cell lines were grown directly on coverslips and fixed with 4% formaldehyde in PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT.
    • Cells were incubated with fluorophore conjugated primary antibody diluted 1:250 and 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
    • Isotype control staining:
    • All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT. - Cells were incubated with 10% normal rabbit serum overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were incubated with goat anti rabbit CY3 conjugated secondary antibody for 60 min in dark at RT.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI. Secondary only staining:
    • All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C. - Cells were incubated with goat anti rabbit CY3 conjugated secondary antibody for 60 min in dark at RT.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
    • Stained cells were imaged with a Nikon C2+ confocal microscope.
    Experimental Notes
    We did the first set of experiment with 1:250 dilution of the primary antibody but have observed lower level of expression of LRP2 in MCF7 (positive) cells (Figure 2). We repeated experiment with higher concentration of the primary antibody (1:100) and the expression level increased as shown in Figure 1. We have noticed lower level of expression of LRP2 in HeLa (negative) cells (Figure 3). We have used rabbit normal serum for isotype control to match the species with primary antibody (rabbit) and also we used bovine albumin serum (BSA) as blocking and antibody diluent buffer to reduce species cross reactivity.
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    LRP2 (Low Density Lipoprotein Receptor-Related Protein 2 (LRP2))
    别名
    Lrp2/Megalin (LRP2 产品)
    别名
    im:680590 antibody, lrp2 antibody, dbs antibody, gp330 antibody, megalin antibody, DBS antibody, GP330 antibody, AI315343 antibody, AW536255 antibody, D230004K18Rik antibody, Gp330 antibody, Megalin antibody, LDL receptor related protein 2 antibody, low density lipoprotein receptor-related protein 2a antibody, low density lipoprotein receptor-related protein 2 antibody, low-density lipoprotein receptor-related protein 2 antibody, LDL receptor related protein 2 L homeolog antibody, LRP2 antibody, lrp2a antibody, LRP2.3 antibody, lrp2.L antibody, LOC100183506 antibody, lrp2 antibody, Lrp2 antibody
    背景

    Synonyms: DBS, GP33, Low-density lipoprotein receptor-related protein 2, LRP-2, Glycoprotein 33, Megalin, LRP2

    Background: Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.

    基因ID
    4036
    UniProt
    P98164
    途径
    Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport
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