Osteoactivin (AA 85-135) 抗体

ABIN749093 产品详细信息, 供应商: Log in to see
抗原
  • HGFIN
  • NMB
  • DC-HIL
  • Dchil
  • ipd
  • glycoprotein (transmembrane) nmb
  • Gpnmb
  • GPNMB
  • gpnmb
抗原表位
AA 85-135
19
18
14
11
9
8
8
7
5
4
4
3
2
2
2
1
1
1
1
1
1
适用
人, 小鼠, 大鼠
121
28
23
5
5
5
4
4
2
2
2
1
1
宿主

68
58
1
克隆类型
多克隆
标记
非结合性
5
5
4
4
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
77
59
37
21
15
12
4
3
3
1
Supplier
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Available images

'独立验证'标志
抗原 Osteoactivin
Method validated Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Positive Control Tumor cell transplanted mouse brain was dissected
Notes The staining pattern was as expected for Osteoactivin as membrane protein.
Primary Antibody ABIN749093
Isotype Control Antibody rabbit IgG
Secondary Antibody Donkey-anti-Rabbit-Cy3
实验流程
  • Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
  • Immunostaining for Cryo Sections:
    • Dry slides at 50-55°C for 15-30 min (or RT for 30min)
    • Wash three times with PBS 5min (shake gently)
    • Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
    • Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
    • Rabbit-anti-Osteoactivin antibody (ABIN749093) diluted 1:50-1:400
    • Wash three times with PBS 5min (shake gently)
    • Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
    • Donkey-anti-Rabbit-Cy3
    • DAPI 1:100
    • Wash three times with PBS 5min (shake gently)
    • Add three drops of water-based Immu-mount
    • Cover with 24x60 cover glass
  • Imaging
    • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
    Experimental Notes ABIN749093 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
    Validation Images
    Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) validation image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) IF validation of osteoactivin antibody ABIN749093 on mouse brain tumor sections. A. A...
    免疫原 KLH conjugated synthetic peptide derived from human GPNMB
    亚型 IgG
    纯化方法 Purified by Protein A.
    别名 Gpnmb
    背景

    The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

    Subcellular location: Extracellular

    Synonyms: NMB, HGFIN, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, UNQ1725/PRO9925

    基因ID 10457
    UniProt Q14956
    研究领域 Cancer, Innate Immunity
    应用备注 WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    限制 仅限研究用
    状态 Liquid
    浓度 1 μg/μL
    缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    储存液 Sodium azide
    注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件 -20 °C
    储存方法 Store at -20°C
    有效期 12 months
    厂商提供的图像
    SDS-PAGE (SDS) image for anti-Osteoactivin (AA 85-135) 抗体 (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Osteoactivin (AA 85-135) 抗体 (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    有引用在: Arosarena, Dela Cadena, Denny, Bryant, Barr, Thorpe, Safadi: "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." in: Journal of cellular physiology, Vol. 231, Issue 8, pp. 1761-70, 2016 (PubMed).

    Frara, Abdelmagid, Tytell, Amin, Popoff, Safadi, Barbe: "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." in: BMC musculoskeletal disorders, Vol. 17, pp. 34, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

    Ruan, Li, Nie, Zhou, Zou: "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4857-66, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

    Abdelmagid, Sondag, Moussa, Belcher, Yu, Stinnett, Novak, Mbimba, Khol, Hankenson, Malcuit, Safadi: "Mutation in Osteoactivin Promotes Receptor Activator of NF?B Ligand (RANKL)-mediated Osteoclast Differentiation and Survival but Inhibits Osteoclast Function." in: The Journal of biological chemistry, Vol. 290, Issue 33, pp. 20128-46, 2015 (PubMed).