Occludin 抗体 (OCLN) (AA 480-520)

Details for Product anti-OCLN Antibody No. ABIN687337, 供应商: Log in to see
抗原
  • tpmt
  • OCLN
  • BLCPMG
  • AI503564
  • Ocl
  • ocln
  • oclnb
  • wu:fd23h10
  • wu:fi13c01
  • zgc:113992
  • zgc:56359
  • occludin
  • occludin a
  • OCLN
  • ocln
  • Ocln
  • ocln-b
  • oclna
Alternatives
anti-小鼠 Occludin 抗体 for Immunofluorescence (fixed cells)
抗原表位
AA 480-520
34
14
13
7
7
6
5
5
4
4
3
3
2
2
1
1
适用
人, 小鼠, 猪, 大鼠
111
55
49
17
16
4
4
4
3
2
2
2
宿主

85
30
3
克隆类型
多克隆
标记
This Occludin antibody is un-conjugated
6
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
100
55
29
25
21
12
10
7
3
1
1
1
Supplier
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Supplier Product No.
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Available images

'独立验证'标志
Lot Number 990698W
Method validated Immunofluorescence
Positive Control Mouse stomach tissue
Negative Control Human fat tissue
Notes Signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antibody: Occludin (OCLN)
  • Catalog number: ABIN687337
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 990698W
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: PPFE mouse stomach tissue
  • Negative Control: PPFE human fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul , Bioss bs-0295p
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only.
实验流程
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Experimental Notes
  • No challenges notes
  • OCLN is recommended to staining of mouse stomach tissue according to this protocol.
Validation Images
Immunofluorescence validation image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (...
免疫原 KLH conjugated synthetic peptide derived from mouse Occludin
亚型 IgG
纯化方法 Purified by Protein A.
别名 Occludin (OCLN Antibody 摘要)
背景

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subcellular location: Cytoplasm

Synonyms: Ocl, AI53564, Occludin, Ocln

基因ID 18260
UniProt Q61146
途径 Cell-Cell Junction Organization, Hepatitis C
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Occludin 抗体 (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (IHC) image for anti-Occludin 抗体 (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
Immunofluorescence (IF) image for anti-Occludin 抗体 (OCLN) (AA 480-520) (ABIN687337) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-Occludin 抗体 (OCLN) (AA 480-520) (ABIN687337) Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin 抗体 (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin 抗体 (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
有引用在: Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, 2015 (PubMed).

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, 2014 (PubMed).

Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, 2013 (PubMed).

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, 2012 (PubMed).

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, 2011 (PubMed).