WNT2B 抗体 (Wingless-Type MMTV Integration Site Family, Member 2B) (AA 341-391)

Details for Product anti-WNT2B Antibody No. ABIN675728, 供应商: Log in to see
抗原
  • wnt2b
  • WNT2B
  • Wnt13
  • WNT13
  • XWNT2
  • XWnt-2
  • Xwnt-2b
  • wnt-2b
  • xwnt2b
  • wingless-type MMTV integration site family, member 2Ba
  • wingless-type MMTV integration site family, member 2B
  • wingless related MMTV integration site 2b
  • wnt2ba
  • WNT2B
  • LOC100227493
  • LOC100349847
  • Wnt2b
  • wnt2b-a
抗原表位
AA 341-391
15
14
7
6
2
2
2
1
1
1
适用
人, 小鼠, 大鼠
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34
32
13
12
11
11
10
9
8
7
5
2
1
1
1
宿主

63
2
克隆类型
多克隆
标记
This WNT2B antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
32
24
14
12
5
3
1
1
1
选项
Supplier
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Supplier Product No.
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'独立验证'标志
Lot Number 120522
Method validated Immunohistochemistry
Positive Control Human kidney
Negative Control Human liver
Notes Faint but distinguishable signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antigen: Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term)
  • Catalog number: ABIN675728
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 120522
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
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  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human kidney tissue stained with antibody
  • Negative control: Human liver tissue stained with antibody
  • Isotype control: Human kidney tissue stained with isotype control
  • Secondary only control: Human kidney tissue stained with secondary antibody only
实验流程
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 1: Human kidney tissue stained with anti-WNT2 (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 2: Human liver tissue stained with anti-WNT2 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 3: Human kidney tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 1: Human kidney tissue stained with secondary antibody only (brown) and counte...
免疫原 KLH conjugated synthetic peptide derived from human WNT2B
亚型 IgG
纯化方法 Purified by Protein A.
别名 Wnt2b (WNT2B Antibody 摘要)
背景

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

Subcellular location: Secreted

Synonyms: WNT13, Protein Wnt-2b, Protein Wnt-13, WNT2B

基因ID 7482
UniProt Q93097
研究领域 Cancer, Wnt
途径 WNT signaling
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months
厂商提供的图像
SDS-PAGE (SDS) image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti WNT2B AVPR...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Independently Validated Antibody, image provided by Science Direct, badge number 0294...
有引用在: Li, Liu, Su, Ren, Zhu, Tian, Qiu: "MicroRNA-324-3p regulates nasopharyngeal carcinoma radioresistance by directly targeting WNT2B." in: European journal of cancer (Oxford, England : 1990), Vol. 49, Issue 11, pp. 2596-607, 2014 (PubMed).

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