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Retinoblastoma 1 抗体 (pSer780)

Name: Retinoblastoma 1 抗体 (pSer780)
SKU: ABIN675294
Availability: OutOfStock
Rating: 5 (1 reviews)

RB1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Retinoblastoma 1 (RB1) (pSer780) antibody (ABIN675294)

1 image

(1 reference)

(1 validation)

产品编号 ABIN675294

发货至: 中国

Datasheet as PDF

抗原 See all Retinoblastoma 1 (RB1) 抗体

Retinoblastoma 1 (RB1)
抗原表位
31

30

29

24

24

22

20

15

11

10

10

7

6

6

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer780
适用
289

113

94

5

4

4

3

3

3

2

1

1

1
人, 大鼠
宿主
250

42
兔
Compatible Secondaries
克隆类型
237

55
多克隆
标记
145

19

17

16

11

11

6

6

6

6

6

6

6

6

6

6

6

1

1

1

1

1

1

1
This Retinoblastoma 1 antibody is un-conjugated
应用范围
221

131

113

79

78

41

39

35

28

18

18

9

8

6

6

2

2
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 大鼠

预测反应

Mouse,Dog,Cow,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human Rb around the phosphorylation site of (Ser780)

亚型

IgG
anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer612) antibody
RB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3C11 unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807) antibody
RB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 5H12 unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807), (pSer811) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (AA 639-778) antibody
RB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029795 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029795

日期

2014.08.21

抗原

Retinoblastoma 1 (RB1) (pSer780)

Lot Number

120508

Method validated

Western Blotting

Positive Control

NIH/3T3 cells irradiated with 25 Joules/m2 UV-C

Negative Control

Non-irradiated NIH/3T3 cells

Notes

A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a lower molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.

Validation Images

Figure 1: Western Blot for RB1 pSer780. Grey arrowhead indicates the expected molecular weight of ~105 kDa.
Full Methods
Primary Antibody

Antigen: Retinoblastoma 1 (RB1) (pSer780)
Catalog number: ABIN675294
Lot number: 120508
Antibody Dilution: 1:100

Secondary Antibody

Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Lot number: L170-6515
Antibody Dilution: 1:10,000

Full Protocol

1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
2. 20 μL of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 20 hours at 4°C.
8. The membrane was rinsed in TBST thrice for 5 minutes each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
11. The membrane was rinsed in TBS twice for 30 seconds each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 50 minutes.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
15. The membrane was washed in TBST 2 times for 10 minutes each.
16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.

Experimental Notes

- No experimental challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Béguelin, Rivas, Calvo Fernández, Teater, Purwada, Redmond, Shen, Challman, Elemento, Singh, Melnick: "EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop." in: Nature communications, Vol. 8, Issue 1, pp. 877, (2018) (PubMed).
抗原

Retinoblastoma 1 (RB1)

别名

Rb/p105-Rb (RB1 产品)

别名

OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody

背景

Synonyms: RbSer780, OSRC, P105 RB, P105RB, PP105, PP110, pRb, RB 1, RB1, RB1 protein, Retinoblastoma 1 including osteosarcoma, Retinoblastoma 1, Retinoblastoma associated protein, Including osteosarcoma, Osteosarcoma, p105-Rb, Rb, RB_HUMAN, Retinoblastoma suspectibility protein, Retinoblastoma-associated protein, Retinoblastoma related osteosarcoma, Retinoblastoma susceptibility gene.
Background: Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

基因ID

5925

途径

Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy