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MME 抗体 (AA 501-600)

Name: MME 抗体 (AA 501-600)
SKU: ABIN669666
Availability: OutOfStock

MME 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (ABIN669666), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated -A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange).

FACS

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD10 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

A549 cells probed with CD10 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary -PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

5 images

(1 reference)

产品编号 ABIN669666

发货至: 中国

Datasheet as PDF

抗原 See all MME 抗体

MME (Membrane Metallo-Endopeptidase (MME))
抗原表位
15

14

10

8

8

7

7

7

7

6

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 501-600
适用
205

57

49

3

2

2

2

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
109

102

2
兔
Compatible Secondaries
克隆类型
108

104

2
多克隆
标记
106

19

18

13

9

6

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MME antibody is un-conjugated
应用范围
130

96

78

66

35

28

21

19

12

12

10

8

5

5

5

3

2

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CD10

亚型

IgG

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MME 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-78 FITC

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6G12E12 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
MME 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 32-164) antibody
MME 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Chopra, Kraus, Schwinn, Ritz, Mattenheimer, Mottok, Rosenwald, Einsele, Beilhack: "Non-invasive bioluminescence imaging to monitor the immunological control of a plasmablastic lymphoma-like B cell neoplasia after hematopoietic cell transplantation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81320, (2013) (PubMed).
抗原

MME (Membrane Metallo-Endopeptidase (MME))

别名

CD10 (MME 产品)

别名

CALLA antibody, CD10 antibody, NEP antibody, SFE antibody, 6030454K05Rik antibody, C85356 antibody, Nep antibody, neprilysin antibody, membrane metalloendopeptidase antibody, membrane metallo endopeptidase antibody, membrane metallo-endopeptidase antibody, MME antibody, Mme antibody

物质类

Chemical

背景

Synonyms: NEP, SFE, CD1, CALLA, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase, Skin fibroblast elastase, MME, EPN
Background: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 3 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

基因ID

4311

UniProt

P08473

途径

RTK signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration