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Hsc70 抗体 (N-Term)

Name: Hsc70 抗体 (N-Term)
SKU: ABIN650754
Availability: OutOfStock

HSPA8 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB17655 unconjugated

Western blot analysis of lysates from A431,Hela,mouse NIH/3T3, rat H-4-II-E cell line (from left to right),using HS Antibody (N-term) (ABIN650754 and ABIN2839488).

Formalin-fixed and paraffin-embedded human brain tissue reacted with HS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

Confocal immunofluorescent analysis of HS Antibody (N-term) (ABIN650754 and ABIN2839488) with cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

HS Antibody (N-term) (ABIN650754 and ABIN2839488) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).

FACS

4 images

(2 references)

产品编号 ABIN650754

发货至: 中国

Datasheet as PDF

抗原 See all Hsc70 (HSPA8) 抗体

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
抗原表位
14

6

5

5

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 82-110, N-Term
适用
83

46

42

21

17

14

13

11

11

10

10

10

5

5

4

4

4

3

3

3

2

1

1

1

1

1
人
宿主
72

27

7

2
兔
Compatible Secondaries
克隆类型
69

38
多克隆
标记
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
应用范围
94

53

40

32

30

29

16

14

11

11

11

5

4

2

2

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

预测反应

B, C, Ha, Hs, M, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8.

克隆位点

RB17655

亚型

Ig Fraction

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HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, PLA, Bind, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (Internal Region) antibody
Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Fitter, Gronthos, Ooi, Zannettino: "The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70." in: Stem cells (Dayton, Ohio), Vol. 35, Issue 4, pp. 940-951, (2017) (PubMed).

Li, Li, Wang, Cui, Gong, Qu, Zhong, Zhou, Zhou, Gao, Li: "Quantitative proteomic study of human prostate cancer cells with different metastatic potentials." in: International journal of oncology, Vol. 48, Issue 4, pp. 1437-46, (2016) (PubMed).
抗原

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

别名

HSPA8 (HSPA8 产品)

别名

hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody

背景

HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

分子量

70898

基因ID

3312

NCBI登录号

NP_006588, NP_694881

UniProt

P11142