Penicillinase 抗体 (HRP)
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- 抗原 See all Penicillinase products
- Penicillinase
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适用
- Enterobacter cloacae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Penicillinase antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- 交叉反应 （详细）
- Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
- 免疫原
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Immunogen: Penicillinase [Enterobacter cloacae]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Penicillinase antibody (Biotin)
适用: Enterobacter cloacae WB, IP, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Application Note: Anti-Penicillinase has been assayed against 1.0 μg of Penicillinase [Enterobacter cloacae] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Penicillinase
- Abstract
- Penicillinase 产品
- 别名
- beta-lactamase TEM-1 antibody, blaTEM-1 antibody
- 背景
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Synonyms: Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody
Background: Penicillinase is a specific type of β-lactamase, showing specificity for penicillins, again by hydrolysing the beta-lactam ring. Molecular weights of the various penicillinases cluster around 50 kDa. Beta-lactamase provides antibiotic resistance by breaking the antibiotics' structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a beta-lactam. Through hydrolysis, the lactamase enzyme breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.
Gene Name: OXA9
- 基因ID
- 9125732
- UniProt
- P05364
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