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- 抗原 See all CPY 抗体
- CPY (Carboxypeptidase Y (CPY))
- 适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CPY antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.
- 纯化方法
- Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker's Yeast].
- 免疫原
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Immunogen: Carboxypeptidase Y [Baker's Yeast]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product CPY Primary Antibody
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anti-Carboxypeptidase Y (CPY) antibody
CPY 适用: Saccharomyces cerevisiae ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Carboxypeptidase Y has been assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- CPY (Carboxypeptidase Y (CPY))
- 别名
- Carboxypeptidase Y (CPY 产品)
- 别名
- AO090103000332 antibody, carboxypeptidase Y antibody, CC1G_12159 antibody, PAS_chr1-4_0013 antibody, MCYG_00135 antibody, MGYG_00701 antibody, AFUA_5G14610 antibody, ANI_1_238044 antibody, AOR_1_542034 antibody
- 背景
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Synonyms: Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C
Background: Carboxypeptidase Y is an enzyme that catalyses the reaction of the release of a C-terminal amino acid with broad specificity. Carboxypeptidase Y is a carboxypeptidase with optimum activity at pH 4.5-6.0. It is inhibited by diisopropyl fluorophosphate.
Gene Name: PRC1
- 基因ID
- 855343
- UniProt
- P00729
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