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- 抗原 See all Collagen IV (COL4) 抗体
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen IV antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL4 Primary Antibody
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig ELISA, WB, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 ELISA, WB, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 1043 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Collagen Type IV Peroxidase Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:2,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:20,000 - 1:100,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL to 20 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- 有效期
- 12 months
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- 抗原
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 别名
- Collagen Type IV (COL4 产品)
- 别名
- collagen, type IV antibody, Let-2 antibody
- 背景
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Synonyms: Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody
Background: Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Gene Name: COL4A4
- 基因ID
- 1282
- UniProt
- P02462
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