COL3 抗体 (HRP)
(1 reference)-
- 抗原 See all COL3 抗体
- COL3 (Collagen, Type III (COL3))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL3 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Anti-Collagen III has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Collagen Type III from human and bovine placenta
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL3 Primary Antibody
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anti-Collagen, Type III (COL3) antibody
COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) (AA 1301-1400) antibodyCOL3 适用: 人, 大鼠, 小鼠, 犬, 兔 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) (AA 801-900) antibodyCOL3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type III (COL3) antibody (Biotin)COL3 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人, 大鼠, 小鼠 WB 宿主: 小鼠 Monoclonal Q76 unconjugated
anti-Collagen, Type III (COL3) antibody (Magnetic Particles)COL3 适用: 人, 大鼠, 小鼠 IP 宿主: 小鼠 Monoclonal Col-29 Magnetic Particles
anti-Collagen, Type III (COL3) antibody (Biotin)COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
anti-Collagen, Type III (COL3) antibody (FITC)COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal FITC
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Collagen Type III Peroxidase Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL to 20 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Increasing the level of cytoskeletal protein Flightless I reduces adhesion formation in a murine digital flexor tendon model." in: Journal of orthopaedic surgery and research, Vol. 15, Issue 1, pp. 362, (2020) (PubMed).
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: "Increasing the level of cytoskeletal protein Flightless I reduces adhesion formation in a murine digital flexor tendon model." in: Journal of orthopaedic surgery and research, Vol. 15, Issue 1, pp. 362, (2020) (PubMed).
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- 抗原
- COL3 (Collagen, Type III (COL3))
- 别名
- Collagen Type III (COL3 产品)
- 别名
- PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, F2 antibody
- 背景
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Synonyms: Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody
Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6 % of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
Gene Name: COL3A1
- 基因ID
- 1281
- UniProt
- P02461
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