电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

His Tag 抗体

WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
产品编号 ABIN559683
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
    请咨询
    宿主
    • 92
    • 56
    • 12
    • 9
    • 8
    • 7
    • 2
    • 1
    小鼠
    克隆类型
    • 105
    • 69
    • 10
    单克隆
    标记
    • 90
    • 22
    • 14
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This His Tag antibody is un-conjugated
    应用范围
    • 152
    • 102
    • 37
    • 27
    • 25
    • 17
    • 14
    • 14
    • 14
    • 13
    • 13
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)
    序列
    HHHHHH
    特异性
    Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
    灵敏度
    WB (with ECL): 1-2ng of His-tagged fusion proteins
    纯化方法
    Protein A affinity chromatography from mouse ascites fluid
    免疫原
    HHHHHH (6x His) synthetic peptide conjugated to KLH
    克隆位点
    HIS-H8
    亚型
    IgG2b
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
    限制
    仅限研究用
  • 浓度
    1mg/mL
    缓冲液
    10mM PBS, pH 7.2, 0.05% NaN3
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

    Chi, Bo, Wu, Jong, Huang: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).

    Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).

    Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, (1998) (PubMed).

    Srinivasula, Ahmad, Fernandes-Alnemri, Alnemri: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, (1998) (PubMed).

  • 抗原
    His Tag
    Abstract
    His Tag 产品
    物质类
    Tag
You are here:
客服