CPA 抗体 (HRP)
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- 抗原 See all CPA products
- CPA (Carboxypeptidase A (CPA))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CPA antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
- 免疫原
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Immunogen: Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Carboxypeptidase A (CPA) antibody
CPA 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A (CPA) antibodyCPA 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Carboxypeptidase A is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be obtained by researchers.
Western Blot Dilution: 1:300 - 1:2,000
ELISA Dilution: 1:3,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- CPA (Carboxypeptidase A (CPA))
- 别名
- Carboxypeptidase A (CPA 产品)
- 背景
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Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.
Gene Name: CPA1
- 基因ID
- 286762
- UniProt
- P00730
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