Pyruvate Kinase 抗体 (HRP)
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- 抗原 See all Pyruvate Kinase (PK) 抗体
- Pyruvate Kinase (PK)
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Pyruvate Kinase antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Pyruvate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].
- 免疫原
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Immunogen: Pyruvate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product PK Primary Antibody
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anti-Pyruvate Kinase (PK) (Center) antibody
PK 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) (AA 476-505), (C-Term) antibodyPK 适用: 人, 小鼠, 大鼠, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) antibodyPK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) antibody (Biotin)PK 适用: 兔 WB, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Pyruvate Kinase (PK) (AA 121-151), (N-Term) antibodyPK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) (AA 476-505), (C-Term) antibodyPK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Pyruvate Kinase has been assayed against 1.0 μg of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Pyruvate Kinase (PK)
- 别名
- Pyruvate Kinase (PK 产品)
- 别名
- CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, PYK3b antibody, PYK3c antibody, Pyruvate kinase antibody, PK antibody, pyruvate kinase antibody, pyruvate kinase (PK) antibody, Pyruvate kinase (PK) antibody, pyruvate kinase PKM antibody, testis expressed 45 antibody, PyK antibody, pykA antibody, PYK3a|PYK3b|PYK3c antibody, pyk antibody, pyk1 antibody, pk antibody, LOC100534543 antibody, TEX45 antibody
- 背景
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Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, HRP
Background: Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.
Gene Name: PKM2
- 基因ID
- 100008676
- UniProt
- P11974
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