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- 抗原 See all Cholesterol Oxidase (choS) products
- Cholesterol Oxidase (choS)
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适用
- Streptomyces
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Cholesterol Oxidase antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Cholesterol Oxidase from other sources is unknown.
- 纯化方法
- Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].
- 免疫原
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Immunogen: Cholesterol Oxidase [Streptomyces]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Cholesterol Oxidase (choS) antibody (Biotin)
choS 适用: Streptomyces ELISA, WB, IP 宿主: 山羊 Polyclonal Biotin
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- 应用备注
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Application Note: Anti-Cholesterol Oxidase has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Cholesterol Oxidase (choS)
- 别名
- Cholesterol Oxidase (choS 产品)
- 别名
- SCD63.13 antibody, cholesterol oxidase antibody, GMC family oxidoreductase antibody, Cholesterol oxidase antibody, choS antibody, SCO4781 antibody, BTH_I0619 antibody, SARE_RS13770 antibody, Lcho_3191 antibody, Npun_R6526 antibody, Amir_2425 antibody, Caci_5961 antibody, Sros_4246 antibody, Kfla_6484 antibody, Snas_3535 antibody, Weevi_2091 antibody
- 背景
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Synonyms: choA antibody, choD antibody, choM antibody, choS antibody, COD antibody
Background: Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.
Gene Name: choA
- 基因ID
- 1215660
- UniProt
- P12676
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