Intestinal Alkaline Phosphatase 抗体
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- 抗原 See all Intestinal Alkaline Phosphatase (ALPI) 抗体
- Intestinal Alkaline Phosphatase (ALPI)
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Intestinal Alkaline Phosphatase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects human Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline phosphatase [human intestine].
- 纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
- Alkaline Phosphatase [Human Intestine].
- Top Product
- Discover our top product ALPI Primary Antibody
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- 应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of AlkalinePhosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 85.0 mg/mL (by Refractometry)
- 缓冲液
- 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % (w/v) Sodium azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- 抗原
- Intestinal Alkaline Phosphatase (ALPI)
- 别名
- Alkaline Phosphatase / ALPI / IAP (ALPI 产品)
- 别名
- IAP antibody, Alp1 antibody, S51097 antibody, 2010001C14Rik antibody, Akp6 antibody, gIAP antibody, alpi antibody, im:7149588 antibody, si:ch211-262h13.1 antibody, wu:fa95f04 antibody, zgc:110794 antibody, ALPI antibody, Alpi antibody, alkaline phosphatase, intestinal antibody, alkaline phosphatase, intestinal, tandem duplicate 1 antibody, intestinal-type alkaline phosphatase antibody, ALPI antibody, Alpi antibody, alpi.1 antibody, LOC100724954 antibody
- 背景
- Synonyms: Intestinal alkaline phosphatase
- 基因ID
- 248
- NCBI登录号
- NP_001622
- UniProt
- P09923
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