Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Internal Region) 抗体

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抗原
  • APPL
  • DIP13alpha
  • 2900057D21Rik
  • 7330406P05Rik
  • AI585782
  • AW209077
  • BB022931
  • C88264
  • DIP13
  • RGD1309388
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • APPL1
  • Appl1
抗原表位
Internal Region
13
9
5
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
适用
牛, 犬, 人, 小鼠, 大鼠
67
32
21
6
4
3
3
3
3
3
1
1
宿主
山羊
47
19
3
克隆类型
多克隆
标记
非结合性
1
1
1
1
1
1
应用范围
Western Blotting (WB)
63
35
18
12
3
3
1
1
1
选项
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免疫原 Peptide with sequence from the internal region of the protein sequence according to NP_036228.1.
序列 C-NRYSRLSKKR ENDK
特异性 This antibody recognizes APPL1.
交叉反应 (详细) Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.
纯化方法 Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
别名 APPL1 / DIP13A (APPL1 Antibody 摘要)
背景 APPL is involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The APPL binds to RAB5A/Rab5 through N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation.Synonyms: Adapter protein containing PH domain, DCC-interacting protein 13-alpha, Dip13-alpha, KIAA1428, PTB domain and leucine zipper motif 1
基因ID 26060, 9606
UniProt Q9UKG1
应用备注 Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in Rat Aortic Smooth SkeletalMuscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for48h (lane 2). Calculated MW of 79.0 kDa according to NP_036228.1. The blot wasco-labeled with Tubulin-specific antibodies as a loading control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 0.5 mg/mL
缓冲液 Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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