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FAS 抗体 (PE)

FAS 适用: 人 FACS 宿主: 小鼠 Monoclonal UB2 PE
产品编号 ABIN5541252
发货至: 中国
  • 抗原 See all FAS 抗体
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This FAS antibody is conjugated to PE
    应用范围
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    Flow Cytometry (FACS)
    特异性
    This antibody recognizes human Fas antigen.
    纯化方法
    Affinity chromatography on protein A
    免疫原
    Recombinant human Fas
    克隆位点
    UB2
    亚型
    IgG1
    Top Product
    Discover our top product FAS Primary Antibody
  • 应用备注
    Flow cytomstry: 20 μL (ready for use). For details see protocol below.
    实验流程
    Flow cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2 % fetal calf serum (FCS) and 0.1 % NaN 3]. 2) Resuspend the cells with washing buffer (5x10 6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 μ L of normal goat serum containing 1 mg/mL normal human IgG and 0.1 % NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 20 μ L of PE labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive control for Flow cytometry transfectant)
    限制
    仅限研究用
  • 状态
    Liquid
    储存条件
    4 °C
    储存方法
    Store at 2-8 °C. Shelf life: one year from despatch.
    有效期
    12 months
  • Mezosi, Wang, Utsugi, Bajnok, Bretz, Gauger, Thompson, Baker: "Induction and regulation of Fas-mediated apoptosis in human thyroid epithelial cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 19, Issue 3, pp. 804-11, (2005) (PubMed).

    Hauptschein, Sloan, Torella, Moezzifard, Giel-Moloney, Zehetmeier, Unger, Ilag, Jay: "Functional proteomic screen identifies a modulating role for CD44 in death receptor-mediated apoptosis." in: Cancer research, Vol. 65, Issue 5, pp. 1887-96, (2005) (PubMed).

    Suhara, Fukuo, Yasuda, Tsubakimoto, Takemura, Kawamoto, Yokoi, Mogi, Kaimoto, Ogihara: "Homocysteine enhances endothelial apoptosis via upregulation of Fas-mediated pathways." in: Hypertension, Vol. 43, Issue 6, pp. 1208-13, (2004) (PubMed).

    Suhara, Kim, Kirshenbaum, Walsh: "Suppression of Akt signaling induces Fas ligand expression: involvement of caspase and Jun kinase activation in Akt-mediated Fas ligand regulation." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 680-91, (2001) (PubMed).

    Samudio, Vyhlidal, Wang, Stoner, Chen, Kladde, Barhoumi, Burghardt, Safe: "Transcriptional activation of deoxyribonucleic acid polymerase alpha gene expression in MCF-7 cells by 17 beta-estradiol." in: Endocrinology, Vol. 142, Issue 3, pp. 1000-8, (2001) (PubMed).

    Sata, Suhara, Walsh: "Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 2, pp. 309-16, (2000) (PubMed).

    Shinohara, Yagita, Ikawa, Oyaizu: "Fas drives cell cycle progression in glioma cells via extracellular signal-regulated kinase activation." in: Cancer research, Vol. 60, Issue 6, pp. 1766-72, (2000) (PubMed).

    Kuwano, Miyazaki, Hagimoto, Kawasaki, Fujita, Kunitake, Kaneko, Hara: "The involvement of Fas-Fas ligand pathway in fibrosing lung diseases." in: American journal of respiratory cell and molecular biology, Vol. 20, Issue 1, pp. 53-60, (1999) (PubMed).

    Hiromatsu, Hoshino, Yagita, Koga, Sakisaka, Honda, Yang, Kayagaki, Okumura, Nonaka: "Functional Fas ligand expression in thyrocytes from patients with Graves' disease." in: The Journal of clinical endocrinology and metabolism, Vol. 84, Issue 8, pp. 2896-902, (1999) (PubMed).

    Dai, Price, Brunner, Krantz: "Fas ligand is present in human erythroid colony-forming cells and interacts with Fas induced by interferon gamma to produce erythroid cell apoptosis." in: Blood, Vol. 91, Issue 4, pp. 1235-42, (1998) (PubMed).

    Boirivant, Pica, DeMaria, Testi, Pallone, Strober: "Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis." in: The Journal of clinical investigation, Vol. 98, Issue 11, pp. 2616-22, (1997) (PubMed).

    Ando, Hiroishi, Kaneko, Moriyama, Muto, Kayagaki, Yagita, Okumura, Imawari: "Perforin, Fas/Fas ligand, and TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5283-91, (1997) (PubMed).

    Hata, Matsuzaki, Takeya, Yoshida, Sonoki, Nagasaki, Kuribayashi, Kawano, Takatsuki: "Expression of Fas/Apo-1 (CD95) and apoptosis in tumor cells from patients with plasma cell disorders." in: Blood, Vol. 86, Issue 5, pp. 1939-45, (1995) (PubMed).

  • 抗原
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    别名
    cd95,fas (FAS 产品)
    别名
    ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
    背景
    It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology to a number of cell-surface receptors, including tumor necrosis factor receptor and nerve growth factor receptor.
    UniProt
    P25445
    途径
    p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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