CAS9 抗体

ABIN5526139 产品详细信息, 供应商: Log in to see
抗原
适用
Streptococcus pyogenes (S. pyogenes)
8
1
宿主
小鼠
5
4
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
1
1
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
9
4
3
3
3
2
1
选项
Supplier
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Supplier Product No.
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原理 Monoclonal antibody to detect Cas9.
免疫原 N-terminal fragment of Streptococcus pyogenes Cas9.
克隆位点 7A9
亚型 IgG1 kappa
纯化方法 Affinity purified using protein G
背景 Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA.
Synonyms: CRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9
基因ID 901176
UniProt Q99ZW2
应用备注 Immunofluorescence: 1:500-1:2,000
Immunoprecipitation: 1:20
Western Blot: 1:1,000
说明

Suggested positive control:
IF, IP, WB

限制 仅限研究用
状态 Liquid
缓冲液 PBS containing 0.02 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 The antibody solution should be gently mixed before use
储存条件 -20 °C
有效期 12 months
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