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- 抗原 See all PUMA (BBC3) 抗体
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PUMA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects PUMA at C-term.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse.
- 纯化方法
- Peptide Affinity Chromatography
- 免疫原
- Synthetic peptide corresponding to 14 amino acids near the carboxy terminus of Human PUMA alpha. .Remarks: The sequence is identical between alpha and beta forms of the PUMA proteins.
- 亚型
- IgG
- Top Product
- Discover our top product BBC3 Primary Antibody
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anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibodyBBC3 适用: 人, 大鼠, Pig WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibodyBBC3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (HRP)BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal HRP
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (FITC)BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal FITC
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (Alkaline Phosphatase (AP))BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (Biotin)BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal PE
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (APC)BBC3 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal APC
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- 应用备注
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ELISA. Western blot: 1 - 2 μg/mL. K562 or 3T3 cell lysate can be used as a positive control and aband at approximately 23 kDa can be detected. A lower band at approximately 16 kDa wasdetected in MOLT4 and U937 cells, which may represent the PUMA-b form. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 缓冲液
- PBS, 0.02 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- 别名
- PUMA (BBC3 产品)
- 背景
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
- 分子量
- 23 kDa
- 基因ID
- 27113
- NCBI登录号
- NP_055232
- UniProt
- Q9BXH1
- 途径
- p53 Pathway, Positive Regulation of Endopeptidase Activity
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