STX1A 抗体
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- 抗原 See all STX1A 抗体
- STX1A (Syntaxin 1A (Brain) (STX1A))
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适用
- 人, 小鼠, 大鼠, Cow, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This STX1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 特异性
- This antibody reacts with 35 kDa membrane protein Syntaxin-1 expressed predominantly in neuronal tissues on Western blotting, Immunoprecipitation and Immunohistochemistry.
- 交叉反应 （详细）
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Species reactivity (expected):Bovine and Rat.
Species reactivity (tested):Human, Mouse and Rat. - 产品特性
- Synonyms: Syntaxin-1A, STX1, Neuron-specific antigen HPC-1
- 纯化方法
- Protein-A Sepharose Chromatography of hybridoma supernatant.
- 免疫原
- Synaptosomal plasma membrane fraction from adult Rat hippocampus. Remarks: This hybridoma was established by fusion of Mouse myeloma cell NSI/Ag4-1 withBalb/c mouse splenocyte.
- 克隆位点
- HPC-1
- 亚型
- IgG1
- Top Product
- Discover our top product STX1A Primary Antibody
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anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibody
STX1A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11-1A8 unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-255) antibodySTX1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibodySTX1A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibodySTX1A 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (N-Term) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (pSer14) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 2-228) antibodySTX1A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-288) antibodySTX1A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: 0.1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 2 μg/200 μL of cell extract. Immunohistochemistry on Paraffin Embedded Sections: 5 μg/mL (Heat treatmentnecessary). Microwave oven: 2 times for 10 minutes each in 10 mM citrate buffer ( pH 6.5). Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold lysisbuffer to make 8 mg/ml solution. 3. Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thickSDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2for 1 hour ina semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). Seethe manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2)for 1 hour at RT or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 mimnutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS-T (5 minutes x 6 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. Remove extra reagent from the membrane bydabbing with paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Thecondition for exposure and development may vary. Positive Controls: Mouse brain, PC12Immunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add primary antibody as suggest in the APPLICATIONS into 200 µL of the supernatant. Mix well and incubate with gentle agitation for 30-120 minutes at 4°C. Add 20 µl of 50%protein A agarose beads resuspended in the cold lysis buffer. Mix well and incubate withgentle agitation for 60 minutes at 4°C.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- STX1A (Syntaxin 1A (Brain) (STX1A))
- 别名
- Syntaxin 1A / STX1A (STX1A 产品)
- 别名
- HPC-1 antibody, P35-1 antibody, STX1 antibody, SYN1A antibody, STX1A antibody, hpc-1 antibody, p35-1 antibody, stx1 antibody, sx1a antibody, syntaxin1a antibody, CG10716 antibody, CG18615 antibody, CG31136 antibody, CG5448 antibody, CT30033 antibody, Dm Syx1 antibody, DmSyx1A antibody, Dmel\\CG31136 antibody, SYX1A antibody, Syt1A antibody, Syx antibody, Syx-1A antibody, Syx1 antibody, Syx1a antibody, SyxA antibody, anon-EST:Gibbs4 antibody, anon-WO02059370.54 antibody, dSyx1 antibody, l(3)06737 antibody, synt antibody, syt-1A antibody, syx antibody, syx-1 antibody, syx-1A antibody, syx1 antibody, syx1A antibody, syntaxin 1A antibody, syntaxin 1A (brain) antibody, Syntaxin 1A antibody, syntaxin-1A antibody, STX1A antibody, Stx1a antibody, stx1a antibody, Syx1A antibody, EDI_005240 antibody, Tsp_07354 antibody
- 背景
- Syntaxin 1, also known as HPC-1, is a 35 kDa integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. Syntaxin is essential for synaptic vesicle fusion and it interacts with several other proteins important for synaptic function, including its partners in the fusion complex (Synaptobrevin/VAMP, SNAP-25, α-SNAP, synaptotagmin, Munc-18/n-Sec1, and Ca2+-channels). Syntaxin is concentrated in synapses and synaptic vesicles where it is a component of the SNARE complex.Synonyms: Neuron-specific antigen HPC-1, STX1, Syntaxin-1A
- 基因ID
- 6804
- UniProt
- Q16623
- 途径
- Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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