山羊 anti-大鼠 IgG1, IgG2a, IgG2b, IgG2c (Fc Region) Antibody
ELISA, DB, IF, WB
Polyclonal
unconjugated
-
- 抗原
- IgG1, IgG2a, IgG2b, IgG2c
-
抗原表位
- Fc Region
- 适用
- 大鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
应用范围
- ELISA, Dot Blot (DB), Immunofluorescence (IF), Western Blotting (WB)
- 特异性
- Purified IgG fraction of polyclonal Goat antiSerum to Rat IgG, Fc specific.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse has been obtained.
- 产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule including all subclasses. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
- 纯化方法
- Purified
- 免疫原
- Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
- 应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
- 抗原
- IgG1, IgG2a, IgG2b, IgG2c
- 别名
- IgG1 IgG2a IgG2b IgG2c
-
You are here:
