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山羊 anti-大鼠 IgA, IgG, IgM (Fab Region) Antibody - Preadsorbed

ELISA, WB, ICC Polyclonal IgG unconjugated
产品编号 ABIN458745
发货至: 中国
  • 抗原 See all IgA, IgG, IgM products
    IgA, IgG, IgM
    抗原表位
    • 75
    • 58
    • 31
    • 16
    • 9
    Fab Region
    适用
    • 72
    • 47
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    大鼠
    宿主
    • 161
    • 55
    • 4
    • 3
    • 1
    山羊
    克隆类型
    • 219
    • 1
    多克隆
    应用范围
    • 117
    • 112
    • 77
    • 73
    • 68
    • 56
    • 26
    • 24
    • 23
    • 22
    • 15
    • 15
    • 13
    • 12
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
    特异性
    The defined antibody specificity is directed to the major isotypes of the rat immunoglobulin system (classes, subclasses and L chain types) including antibody to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony).
    产品特性
    Purified IgG fraction of polyclonal goat antiserum to rat immunoglobulins IgG, IgA, IgM, heavy and light chains
    纯化方法
    Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted rat serum as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
    免疫原
    Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
    亚型
    IgG
  • 应用备注
    The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
    The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    浓度
    10 mg/mL
    缓冲液
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    储存液
    Without preservative
    注意事项
    Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refroze n, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • 抗原
    IgA, IgG, IgM
    别名
    IgG + IgA + IgM (IgA, IgG, IgM 产品)
    物质类
    Antibody
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