山羊 anti-大鼠 IgA (Fc Region) Antibody
ELISA, WB, IF, DB
Polyclonal
unconjugated
-
- 抗原 See all IgA products
- IgA
-
抗原表位
- Fc Region
-
适用
- 大鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
- 特异性
- IgG fraction of polyclonal Goat antiSerum to Rat IgA, Fc specific.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail, however in double radial immunodiffusion a weak reaction with Mouse has been observed.
- 产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgM and IgG/Fab or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
- 纯化方法
- Purified
- 免疫原
- Purified homogenous IgA isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
Goat anti-Rat IgA (Heavy Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Biotin
-
- 应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
-
You are here:
