(1 reference)-
- 抗原 See all IgG1 products
- IgG1
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- The defined antibody specificity is directed to IgG1 as tested against mouse sera and a panel of purified homogenous immunoglobulins and at the level of sensitivity of direct immunofluorescence staining methods applied to lymphoid cells with the conjugated form of this product. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against mouse serum and the purified monoclonal IgG1
- 产品特性
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Purified IgG fraction of polyclonal rabbit antiserum to mouse IgG1, subclass specific
Performance testing Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG(7S) is further evaluated in a number of highly sensitive non-precipitating antibody-binding as - 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
- 免疫原
- Pools of highly purified homogenous IgG1 isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody - PreadsorbedELISA, WB, IHC Polyclonal IgG unconjugated
Rat anti-Mouse IgG1 antibodyELISA, FACS Monoclonal SB77e IgG2b unconjugated
Rat anti-Mouse IgG1 antibody (HRP)ELISA Monoclonal SB77E IgG2b HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)ELISA, WB, IHC, FACS Polyclonal IgG Biotin
Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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The cytochemical grade of RAM/IgG1/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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: "A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line." in: Malaria journal, Vol. 18, Issue 1, pp. 313, (2019) (PubMed).
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: "A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line." in: Malaria journal, Vol. 18, Issue 1, pp. 313, (2019) (PubMed).
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- 抗原
- IgG1
- Abstract
- IgG1 产品
- 物质类
- Antibody
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