兔 anti-小鼠 IgA (Fc Region) Antibody (FITC)
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- 抗原 See all IgA products
- IgA
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抗原表位
- Fc Region
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse IgA Fc specific
- 交叉反应 （详细）
- This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.
- 产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against mouse serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react IgG, IgG/Fab fragments and IgM or any non-Ig protein in mouse serum, as tested by immuno-electrophoresis nd double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of mouse origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- 纯化方法
- Purified
- 免疫原
- Pools of purified pools of homogenous IgA isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Mouse IgA (Heavy Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgA (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rat anti-Mouse IgA antibody (PE)ELISA, FACS Monoclonal 11-44-2 IgG1 PE
Rat anti-Mouse IgA (Heavy Chain) antibody (HRP)ELISA, IHC Monoclonal 11-44-2 IgG1 HRP
Goat anti-Mouse IgA (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rat anti-Mouse IgA antibodyELISA, FACS Monoclonal 11-44-2 IgG1 unconjugated
Goat anti-Mouse IgA (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Rat anti-Mouse IgA antibody (Biotin)ELISA, FACS Monoclonal 11-44-2 IgG1 Biotin
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- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
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