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山羊 anti-小鼠 IgA, IgG, IgM (Fab Region) Antibody (FITC) - Preadsorbed

IF Polyclonal IgG FITC
产品编号 ABIN458392
发货至: 中国
  • 抗原 See all IgA, IgG, IgM products
    IgA, IgG, IgM
    抗原表位
    • 75
    • 57
    • 31
    • 16
    • 9
    Fab Region
    适用
    • 72
    • 46
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    小鼠
    宿主
    • 160
    • 55
    • 4
    • 3
    • 1
    山羊
    克隆类型
    • 218
    • 1
    多克隆
    标记
    • 54
    • 38
    • 33
    • 27
    • 22
    • 11
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    FITC
    应用范围
    • 117
    • 112
    • 77
    • 72
    • 68
    • 56
    • 26
    • 24
    • 23
    • 22
    • 15
    • 15
    • 13
    • 12
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF)
    特异性
    The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
    产品特性
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to mouse IgG, IgA and IgM, heavy and light chains, adsorbed for human cross-reactivity.
    Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7.
    纯化方法
    Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted mouse.
    免疫原
    Purified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
    亚型
    IgG
  • 应用备注
    Direct immunofluorescence staining of monoclonal mouse monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    浓度
    10 mg/mL
    缓冲液
    FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    储存液
    Without preservative
    注意事项
    Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • 抗原
    IgA, IgG, IgM
    别名
    IgG + IgA + IgM (IgA, IgG, IgM 产品)
    物质类
    Antibody
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