山羊 anti-小鼠 IgE (Fc Region) Antibody (TRITC)
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- 抗原 See all IgE products
- IgE
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抗原表位
- Fc Region
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgE, Fc specific
- 交叉反应 （详细）
- This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this conjugate has been passed over appropriate immuno-adsorbents to remove antibodies cross-reacting with Mouse immunoglobulins. This renders it specific for use in test systems containing material of Mouse origin (e.g. Mouse tissue/Mouse monoclonal antibody to a Mouse tissue constituent/anti Mouse Ig isotype-specific immunoconjugate.
- 产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- 纯化方法
- Purified
- 免疫原
- Pools of purified homogenous IgE isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Mouse IgE (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP
Rat anti-Mouse IgE (Heavy Chain) antibody (HRP)ELISA, IHC Monoclonal 23G3 IgG1 HRP
Goat anti-Mouse IgE antibody (PE)ELISA, FLISA Polyclonal IgG PE
Goat anti-Mouse IgE (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgE (Heavy Chain) antibodyELISA, WB, IHC Polyclonal IgG unconjugated
Rat anti-Mouse IgE antibody (Biotin)ELISA Monoclonal 23G3 IgG1 Biotin
Rat anti-Mouse IgE antibody (FITC)ELISA Monoclonal 23G3 IgG1 FITC
Rat anti-Mouse IgE (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC Monoclonal 23G3 IgG1 Alkaline Phosphatase (AP)
Goat anti-Mouse IgE (Heavy Chain) antibody (Biotin)ELISA, WB, IHC Polyclonal IgG Biotin
Goat anti-Mouse IgE antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
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- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- IgE
- Abstract
- IgE 产品
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