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山羊 anti-人 IgD (Fc Region) Antibody

ELISA, IF, WB, DB Polyclonal unconjugated
产品编号 ABIN458224
发货至: 中国
  • 抗原 See all IgD products
    IgD
    抗原表位
    • 16
    • 13
    • 5
    • 1
    Fc Region
    适用
    • 36
    • 11
    • 3
    宿主
    • 22
    • 11
    • 7
    • 5
    • 5
    山羊
    克隆类型
    • 34
    • 15
    多克隆
    应用范围
    • 28
    • 21
    • 13
    • 10
    • 9
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    ELISA, Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB)
    特异性
    Specificity Reference Reagent. Purified IgG fraction of polyclonal Goat antiSerum to Human IgD, Fc specific.
    交叉反应 (详细)
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail because of the low level of IgD in most species.
    产品特性
    The reactivity of the antiserum is directed to the Fc subunits of the IgD molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgD, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum. As unlabelled secondary antibody for indirect detection of IgD in human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
    纯化方法
    Purified
    免疫原
    Purified homogenous IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • 应用备注
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
    缓冲液
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
    储存液
    Without preservative
    储存条件
    RT,4 °C,-20 °C
    储存方法
    The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • 抗原
    IgD
    Abstract
    IgD 产品
    物质类
    Antibody
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