山羊 anti-人 Ig lambda (Free & Bound) Antibody (FITC)
ELISA, ICC, IF, IHC (fro)
Polyclonal
IgG
FITC
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- 抗原 See all Ig lambda products
- Ig lambda
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抗原表位
- Free & Bound
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Specificity Reference Reagent Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to free and bound Human Ig lambda light chain.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
- 产品特性
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The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasma. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Clone: Polyclonal - 纯化方法
- Purified
- 免疫原
- A pool of purified Bence Jones lambda proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Fluorochrome coupled purified goat IgG lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Fluorochrome coupled purified goat IgG lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- Ig lambda
- Abstract
- Ig lambda 产品
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