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小鼠 anti-人 IgG2 (Chain kappa) Antibody

ELISA, WB, IF Monoclonal NI 6014 (HP 6014) IgG1 kappa unconjugated
产品编号 ABIN457929
发货至: 中国
  • 抗原 See all IgG2 products
    IgG2
    抗原表位
    • 14
    • 6
    • 2
    • 1
    • 1
    • 1
    Chain kappa
    适用
    • 20
    • 13
    • 7
    • 7
    • 7
    • 1
    宿主
    • 17
    • 11
    • 11
    • 10
    • 6
    小鼠
    克隆类型
    • 32
    • 23
    单克隆
    应用范围
    • 29
    • 28
    • 26
    • 22
    • 14
    • 8
    • 8
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    特异性
    The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG2, subclass specific
    纯化方法
    Delipidated, heat inactivated lyophilized stable ascites
    免疫原
    Highly purified monoclonal IgG1 kappa isolated from pooled human serum
    克隆位点
    NI 6014 (HP 6014)
    亚型
    IgG1 kappa
  • 应用备注
    To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
    The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
    浓度
    1.0 mg/mL
    缓冲液
    No foreign proteins added.
    储存液
    Without preservative
    注意事项
    Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • 抗原
    IgG2
    Abstract
    IgG2 产品
    物质类
    Antibody
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