兔 anti-Cow IgM (Fc Region) Antibody
ELISA, WB, IF, DB
Polyclonal
unconjugated
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- 抗原 See all IgM products
- IgM
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抗原表位
- Fc Region
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
- 特异性
- IgG fraction of polyclonal Rabbit antiSerum to Bovine IgM, Fc specific.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
- 产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
- 纯化方法
- Purified
- 免疫原
- Purified normal IgM isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- 应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
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