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RFP 抗体 (Red Fluorescent Protein) (Atto 542)

Details for Product anti-RFP Antibody No. ABIN4368321, 供应商: Log in to see
抗原
适用
Discosoma
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宿主
Camelid (Camelidae)
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克隆类型 (克隆位点)
单克隆 ()
标记
This RFP antibody is conjugated to Atto 542
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应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF)
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Supplier
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原理 FluoTag-Q series - high affinity monovalent binders designed for quantitative imaging
品牌 FluoTag-Q
免疫原 RFP
克隆位点 2B12
片段 VHH fragment
特异性 Most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato
交叉反应 (详细) Does not cross-react with GFP, mTagBFP or mTagRFP or their most common derivatives
产品特性 FluoTag-Q anti-RFP is derived from an in-house developed single-domain antibody (sdAb) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95 % guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
别名 RFP
背景 Red fluorescent protein RFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse RFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of RFP fusion proteins can be exceeded by far.
分子量 26 kDa
研究领域 Tags/Labels
应用备注 1:1000 (IF, ICC)
限制 仅限研究用
状态 Liquid
浓度 5 μM
缓冲液 PBS, 50 % glycerol, 0.09 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Protect from light!
储存条件 -20 °C
储存方法 Up to 3 months: -20 °C Up to 12 months: -80 °C or below
厂商提供的图像
Immunofluorescence (IF) image for anti-RFP 抗体 (Red Fluorescent Protein)  (Atto 542) (ABIN4368321) 3T3 cells transfected with the mCherry-tubulin and stained with FluoTag-Q anti-RFP At...