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GFP 抗体 (Green Fluorescent Protein) (Abberior STAR 635P)

Details for Product anti-GFP Antibody No. ABIN4368313, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
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宿主
Camelid (Camelidae)
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克隆类型 (克隆位点)
单克隆 ()
标记
This GFP antibody is conjugated to Abberior STAR 635P
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应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF)
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原理 FluoTag-X series - Multiple fluorophores per affinity probe and multiple affinity probes per target molecule resulte in maximal brightness
品牌 FluoTag-X
免疫原 GFP
克隆位点 1H1-1B2
片段 VHH fragment
特异性 GFP, mEGFP, sfGFP and most common CFP and YFP variants
交叉反应 (详细) Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
产品特性 FluoTag-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our FluoTag-X4 products excellent tools for all microscopy techniques.
别名 GFP
背景 Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse GFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of GFP fusion proteins can be exceeded by far.
分子量 27 kDa
研究领域 Tags/Labels
应用备注 1:250 (IF,ICC)
限制 仅限研究用
状态 Liquid
浓度 2.5 μM
缓冲液 PBS, 50 % glycerol, 0.09 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Protect from light!
储存条件 -20 °C
储存方法 Up to 3 months: -20 °C Up to 12 months: -80 °C or below
厂商提供的图像
Immunofluorescence (IF) image for anti-GFP 抗体 (Green Fluorescent Protein)  (Abberior STAR 635P) (ABIN4368313) HeLa cells transfected with the nuclear pore complex marker GFP-Nup98 stained with Fl...