Myc 标记 抗体 (HRP)

ABIN401754 产品详细信息, 供应商: Log in to see
抗原
适用
标记
217
41
14
12
6
5
3
1
1
宿主

164
63
21
17
3
2
1
克隆类型
多克隆
标记
HRP
27
24
15
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
227
121
83
54
52
39
23
19
18
7
6
5
5
5
5
4
3
3
2
2
1
1
1
1
1
1
1
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
免疫原 Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
序列 EQKLISEEDL
特异性 This antibody is directed against human c-myc.
产品特性 Absorption / Emission: 770 nm / 794 nm
Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG
纯化方法 Affinity chromatography
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag
基因ID 4609
NCBI登录号 NP_002458
UniProt P01106
研究领域 Transcription Factors, Cancer, Cell Cycle
应用备注 ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.
限制 仅限研究用
溶解方式 Restore with 0.1 mL of deionized water (or equivalent).
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative
储存液 Gentamicin sulfate
注意事项 Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
您还需要查找其他产品吗?