Myc Tag 抗体 (HRP)
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- 抗原 See all Myc Tag products
- Myc Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Myc Tag antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 序列
- EQKLISEEDL
- 特异性
- This antibody is directed against human c-myc.
- 产品特性
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Absorption / Emission: 770 nm / 794 nm
Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG - 纯化方法
- Affinity chromatography
- 免疫原
- Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
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anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag antibodyWB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-Myc Tag (AA 408-439) antibody适用: 人 WB, IP, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody
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- 应用备注
- ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative
- 储存液
- Gentamicin sulfate
- 注意事项
- Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Myc Tag
- 别名
- C-Myc Epitope Tag (Myc Tag 产品)
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag
- 基因ID
- 4609
- NCBI登录号
- NP_002458
- UniProt
- P01106
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