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Myc Tag 抗体 (Biotin)

WB, IHC (fro), EIA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN401744
发货至: 中国
  • 抗原 See all Myc Tag products
    Myc Tag
    适用
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    标记
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    This Myc Tag antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    序列
    EQKLISEEDL
    特异性
    This antibody is directed against human c-myc.
    产品特性
    Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
    纯化方法
    Affinity chromatography
    免疫原
    Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
  • 应用备注
    ELISA: 1/4,000 - 1/20,000. Western Blot: 1/400 - 1/2,000. Immunohistochemistry: 1/400 - 1/2,000.
    限制
    仅限研究用
  • 溶解方式
    Restore with 0.1 mL of deionized water (or equivalent). For extended storage mix with glycerol to 50 % .
    浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as perservative
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    This product is photosensitive and should be protected from light
    储存条件
    4 °C
    储存方法
    Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!
  • 抗原
    Myc Tag
    别名
    C-Myc Epitope Tag (Myc Tag 产品)
    物质类
    Tag
    背景
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag
    基因ID
    4609
    NCBI登录号
    NP_002458
    UniProt
    P01106
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