Myc 标记 抗体 (Biotin)

ABIN401744 产品详细信息, 供应商: Log in to see
抗原
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标记
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克隆类型
多克隆
标记
Biotin
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应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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免疫原 Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
序列 EQKLISEEDL
特异性 This antibody is directed against human c-myc.
产品特性 Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
纯化方法 Affinity chromatography
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag
基因ID 4609
NCBI登录号 NP_002458
UniProt P01106
研究领域 Transcription Factors, Cancer, Cell Cycle
应用备注 ELISA: 1/4,000 - 1/20,000. Western Blot: 1/400 - 1/2,000. Immunohistochemistry: 1/400 - 1/2,000.
限制 仅限研究用
溶解方式 Restore with 0.1 mL of deionized water (or equivalent). For extended storage mix with glycerol to 50 % .
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as perservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!
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