SAE1 抗体 (Ser185)
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- 抗原 See all SAE1 抗体
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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抗原表位
- Ser185
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适用
- 人, 小鼠, 大鼠, Cow, 犬, Chimpanzee
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SAE1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against SUMO Activating Enzyme E1 protein.
- 交叉反应 （详细）
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Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 免疫原
- Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein
- 亚型
- IgG
- Top Product
- Discover our top product SAE1 Primary Antibody
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anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibody
SAE1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G4-1G5 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodyKO Validated SAE1 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Internal Region) antibodySAE1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibodySAE1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibodySAE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2887-RB2888 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibodySAE1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 9D8 unconjugated Recombinant Antibody
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Center) antibodySAE1 适用: 人, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-100) antibodySAE1 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/100,000 - 1/125,000. Western Blot: 1/750 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.71 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- 别名
- SAE1 / AOS1 (SAE1 产品)
- 别名
- AOS1 antibody, HSPC140 antibody, SUA1 antibody, UBLE1A antibody, 2400010M20Rik antibody, 2610044L12Rik antibody, AL033372 antibody, AW743391 antibody, D7Ertd177e antibody, Sua1 antibody, Uble1a antibody, Aos1p antibody, Sua1p antibody, aos antibody, sae2a antibody, uble1a antibody, wu:fa28b04 antibody, zgc:86633 antibody, SUMO1 activating enzyme subunit 1 antibody, SUMO1 activating enzyme subunit 1 L homeolog antibody, SAE1 antibody, Sae1 antibody, sae1.L antibody, sae1 antibody
- 背景
- SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
- 基因ID
- 10055
- NCBI登录号
- NP_005491
- UniProt
- Q9UBE0
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