This antibody is directed against beta Actin protein. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
纯化方法
Affinity Chromatography.
免疫原
Synthetic peptide corresponding to amino acids 359-368 of Human beta Actin
ACTB
适用: 人, 小鼠, 大鼠
WB, IF, IHC (p), FACS, DNA Mic
宿主: 小鼠
Monoclonal
137CT26-1-1
unconjugated
应用备注
This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry andwestern blot. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western Blot: 1/1,000-1/4,000. Immunofluorescence: 1/500-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.02 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody at-20 °C. Dilute only prior to immediate use.
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.Synonyms: Actin cytoplasmic 1, Beta-Actin