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V5 Epitope Tag 抗体

WB, EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN401225
发货至: 中国
  • 抗原 See all V5 Epitope Tag products
    V5 Epitope Tag
    适用
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    宿主
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    克隆类型
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    标记
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    This V5 Epitope Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA)
    序列
    GKPIPNPLLG LDST
    纯化方法
    Affinity chromatography
    免疫原
    Peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide
    亚型
    IgG
  • 应用备注
    ELISA: 1: 10,000 - 1: 60,000. Western blot: 1: 5,000 - 1: 10,000. Immunohistochemistry: 1: 500 - 1: 3,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
  • 抗原
    V5 Epitope Tag
    别名
    Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag 产品)
    物质类
    Tag
    背景
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
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