V5 Epitope 标记 抗体

ABIN401225 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主

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克隆类型
多克隆
标记
非结合性
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应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide
序列 GKPIPNPLLG LDST
亚型 IgG
纯化方法 Affinity chromatography
别名 Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
物质类 Tag
背景 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
研究领域 Tags/Labels
应用备注 ELISA: 1: 10,000 - 1: 60,000. Western blot: 1: 5,000 - 1: 10,000. Immunohistochemistry: 1: 500 - 1: 3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
厂商提供的图像
 image for anti-V5 Epitope Tag antibody (ABIN401225) anti-V5 Epitope Tag antibody
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